目的 优选菟丝子总黄酮的最佳提取方法,并对提取物的雌激素活性进行测定。方法 用紫外分光光度法测定菟丝子总黄酮的含量,并以此作为评价指标,采用L4(23)正交设计实验对超声提取、回流提取及闪式提取法进行工艺优化和筛选;并采用性未成熟小鼠子宫增重和子宫(苏木精-伊红)(HE)染色法,以小鼠的子宫系数和子宫内膜厚度评价菟丝子雌激素活性。结果 在3种提取方法中,闪式提取法用时最短,总黄酮含量最高。其提取工艺为体积分数70%乙醇作为提取溶剂,料液比1∶40,累计提取时间2 min,提取电压100 V。雌激素活性研究结果显示,菟丝子总黄酮提取物可明显促进性未成熟小鼠子宫增重,子宫内膜厚度增加。结论 闪式提取法节能高效,适用于菟丝子总黄酮的提取。菟丝子总黄酮提取物具有较好的雌激素活性,可为菟丝子健康功能产品的合理开发和利用提供参考。
Abstract
OBJECTIVE To select the best extraction method for total flavonoids in Cuscuta chinensis and determine the estrogenic activity of the extract. METHODS A method for the content determination of total flavonoids in Cuscuta chinensis was established by UV spectrophotometry. The orthogonal design L4(23) was used to optimize and screen the ultrasonic extraction, reflux extraction and flash extraction with the content of total flavonoids as the evaluation index. The uterine coefficient and endometrial thickness were used to evaluate the estrogenic activity of Cuscuta chinensis in immature mice. RESULTS The flash extraction method showed the shortest time and highest total flavonoids content among the three extraction methods. The extraction process was as follows: 70% ethanol as the extraction solvent, the ratio of material to liquid of 1∶40, the cumulative extraction time of 2 minutes and the extraction voltage of 100 V. The results of estrogen activity study showed that the total flavonoids in Cuscuta chinensis could significantly increase uterine weight and endometrial thickness in immature mice. CONCLUSION The flash extraction method is energy efficient and suitable for the extraction of total flavonoids in Cuscuta chinensis. The total flavonoids in Cuscuta chinensis has good estrogenic activity, which can provide a reference for the rational development and utilization of Cuscuta chinensis functional products.
关键词
菟丝子 /
总黄酮 /
回流提取 /
超声提取 /
闪式提取 /
雌激素活性
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Key words
Cuscuta chinensis /
total flavonoid /
reflux extraction /
ultrasonic extraction /
flash extraction /
estrogenic activity
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中图分类号：
R284.2
R285.5
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脚注
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基金
黑龙江省普通本科高等学校青年创新人才培养计划项目资助(UNPYSCT-2018135);大学生创新创业训练计划项目资助(201910240010)
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